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Terrence Kurzynski's Career History

Microbiology Consultant: 2005-Present

1994-2005 Advanced Microbiologist, Wisconsin State Laboratory of Hygiene (WSLH), Madison, WI.
Duties have largely (75%) included being the lead person for developing and performing molecular subtyping using pulsed field gel electrophoresis for a variety of foodborne and other pathogens. These include: E. coli O157:H7, Salmonella, many serotypes, Listeria monocytogenes, Campylobacter jejuni, Shigella species, methicillin resistant Staphylococcus aureus (MRSA), Legionella pneumophila, Neisseria meningitidis and miscellaneous other pathogens. I was certified by the CDC to perform the PulseNet Standard PFGE procedures for E. coli O157:H7, Salmonella serotypes, L. monocytogenes, Campylobacter and Staphylococcus aureus and to have electronic access to the respective PulseNet BioNumerics databases.

In addition, I was the WSLH's lead person for performing Level B conventional laboratory testing methods for the culture and identification of agents of bioterrorism. These include Bacillus anthracis, Yersinia pestis, Franciscella tularensis, Brucella species and Burkeholderia pseudomallei and B. mallei. Utilized conventional CDC recommended methods for the identification of unusual pathogens referred to our state reference lab. In 2002 I began to use the 16S rDNA gene sequencing methods for clinical and environmental bacterial isolate identification. I have also performed PCR for the detection of stx1, stx2 and eae genes that are virulence factors in pathogenic E. coli strains and I have knowledge using "real time" PCR with the Light Cycler (Roche). I taught other personnel as well as many students in 3 different advanced courses at the UW-Madison. Over my career, I have been a co-author on 29 abstracts presented at scientific meetings and I presented 10 of them. I also have been an author on 29 publications in peer reviewed journals and I was the senior author for 10 of these publications I have successfully worked with UW- Madison faculty members, researchers at the CDC and epidemiologists at the Wisconsin Bureau of Public Health. I have directed the work of various personnel and students over my entire career at the WSLH. My research has validated the reliability of many of the methods used in the WSLH bacteriology laboratory.

1992-1994 Academic Staff, (WSLH).
Presented 10 lectures in UW-M course entitled: "Public Health and Clinical Microbiology", Preventive Medicine course #603. Also presented all course laboratory sessions and worked on various research projects including use of Flow Cytometry for performing antibiotic susceptibility testing on Mycobacterium species.

1979-1992 Supervisor, General Bacteriology Unit, WSLH.
Supervised 7 microbiologists in performing culture, identification and antibiotic susceptibility testing for aerobic, facultative and anaerobic bacterial pathogens. Used CDC conventional and VPI anaerobic techniques for identification. Also introduced use of tissue culture toxin assay and anaerobic culture for detection of Clostridium difficile in stool specimens. Performed research studies in various areas related to clinical microbiology testing, presented and published results from these studies.

1972-1979 Assistant Supervisor, General Bacteriology Unit, WSLH. Worked on culture, identification and antibiotic susceptibility testing for aerobic, facultative and anaerobic bacterial pathogens. Also trained many new personnel, Medical Technology students, resident pathologists and Infectious Disease Fellows from the UW. Presented workshops on various laboratory techniques to bench microbiologists from throughout WI and other states.

1969-1972 Research Microbiologist, St. Mary's Hospital, Burn Research Laboratory, Milwaukee, WI.
Worked on use of vaccines for Pseudomonas aeruginosa in burn and cystic fibrosis patients.

 

Workshops Attended (Limited to last 11 years)

1994   PCR Methods—4 day course, Madison Area Technical College

1996   One week course entitled "Standardized Molecular Subtyping of E. coli 0157:H7 by PFGE", CDC, Atlanta, GA.

1997   Three day follow-up course on PFGE, CDC, Atlanta, Georgia.

1997   One day seminar on investigating indoor bioaerosol contamination, present by Dr. Patricia Hensohn.

1998   Three day Molecular Analyst Finger Printing Plus DST software training at BioRad in Hercules, CA.

1999   Three day BioNumerics software training, Atlanta, GA.

2000   Five day Agents of Bioterrorism Level B Laboratory Training at the CDC in Atlanta, GA.

2000   Two day Advanced BioNumerics software training, San Antonio, TX

1997-2005   Attended all 9 Annual PulseNet Update meetings.

 

Workshops Presented

  1. General Medical Bacteriology—1 week course.*
  2. Anaerobic Bacteriology—1 week course.
  3. Practical Methods in Anaerobic Bacteriology—3 day course.*
  4. Antibiotic Susceptibility Testing—3 day course.
  5. Current Topics in Clinical Bacteriology—1 day course.
  6. Use of Commercial Kit Systems for Identification of Non-fermentative Gram Negative Rods—2 day course.
  7. CDC Level B Agents of Bioterrorism Laboratory Training course, presented one time at the state of GA Public Health Lab and twice at the State of VA Public Health Lab in Richmond, VA in 2004/05. Presented lectures on laboratory testing methods for Bacillus anthracis, Yersinia pestis and Franciscella tularensis. Also presented laboratory sessions concerning these same agents and assisted in presenting laboratory sessions for Brucella species and Burkeholderia pseudomallei and B. mallei.

* Presented multiple times.

 

Membership in Professional Organizations

American Society for Microbiology

PulseNet (prior to retirement)

 

Awards

Recipient of a State of Wisconsin Merit Award for evaluating and implementing the use of selective throat culture media for Group A Streptococcus. At the time we were performing about 35,000 throat cultures per year and the new method saved us many thousands of dollars per year. Also received a Governor's Special Citation for this same work.

Recipient of the 2002 PulseStar award from the Centers for Disease Control for exceptional contributions made the National Molecular Subtyping Network for Foodborne Disease Surveillance. Award consisted of a plaque and $500.

 

Miscellaneous Accomplishments

I served on the National Committee for Clinical Laboratory Standards (NCCLS) Subcommittee on Anaerobic Bacteria which published the following: Reference Agar Dilution Procedure for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Alternative Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria.

From 1984-1986 I was an active participant in the Center for Disease Control Multicenter Pertussis Surveillance Project, and published two manuscripts as a result of the project.

I have served as a reviewer for manuscripts submitted to the European Journal of Clinical Microbiology and Archives of Ophthalmology.

I was chosen by subject matter experts at the Centers for Disease Control in Atlanta, GA to present lectures and laboratory sessions on LRN Level B Agents of Bioterrorism Laboratory testing methods in 2004-2005 for Bacillus anthracis (one class), Francisella tularensis (2 classes), and Yersinia pestis (2 classes). The 4.5 day course was held once in Atlanta, GA and twice in Richmond, VA at the respective state public health laboratories. There were 16 students, mostly from state public health laboratories, in each class.

 

Certifications

During employment at the WSLH, I was certified by the Centers for Disease Control in Atlanta, Ga. to perform the PulseNet Standard PFGE procedures for E. coli O157:H7, Salmonella serotypes, Listeria monocytogenes, Campylobacter jejuni and Staphylococcus aureus and to have electronic access to the PulseNet BioNumerics databases.

 
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